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Recombinant Mouse Kitl (26-189aa)

Cat. No.: Kitl-042E
Product Overview: Recombinant Mouse Kitl (Lys26 ­ Ala189, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.
Description: Stem cell factor (SCF), also known as c­kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 ­ 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Source: E. coli
N­terminal Sequence Analysi: Met
Molecular Mass: 18.6 kDa
Activity: Measured in a cell proliferation assay using TF­1 human erythroleukemic cells. The ED50 for this effect is typically 2.5 ­ 10 ng/mL.
Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 200 μg/mL in PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
Unitprot ID: P20826
Gene Name: Kitl kit ligand [ Mus musculus ]
Official Symbol: Kitl
Synonyms: Kitl; kit ligand; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; Kitlg; contrasted; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL
Gene ID: 17311
mRNA Refseq: NM_013598
Protein Refseq: NP_038626
Chromosome Location: 10 D1; 10 57.0 cM
Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Kit Receptor Signaling Pathway; Melanogenesis; Pathways in cancer
Function: cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding

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