Expand Expand| Cat. No. : | IFNA2-02H |
| Product Overview : | Recombinant Human Interferon alpha-2a produced inE. coliis a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19376 Dalton. Pegylated IFN alpha-2a is manufactured by attaching a 40KD NHS-mPEG to the primary amino acid of IFN alpha-2a. |
| Description : | At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end. |
| Purity : | >98% as determined by HPLC, FPLC and SDS-PAGE analysis. |
| Formulation : | Lyophilized from a (1mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer. |
| Specific Activity : | PEG-IFNα2a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0 x 106IU/mg. |
| Endotoxin : | Less than 0.1 ng/µg (1IEU/µg) of PEG-IFN alpha-2a. |
| Reconstitution : | It is recommended to reconstitute the lyophilized PEG-IFN alpha-2a in sterile 18M.-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : | Lyophilized PEG-IFN alpha-2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PEG-IFN alpha-2a should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Gene Name : | IFNA2 |
| Synonyms : | IFNA; INFA2; MGC125764; MGC125765; Leukocyte interferon; B cell interferon, Type I interferon; IFNA2; IFN-α |
| Gene ID : | 3440 |
| mRNA Refseq : | NM_000605 |
| Protein Refseq : | NP_000596 |
| MIM : | 147562 |
| UniProt ID : | P01563 |
| Chromosome Location : | 9p22 |
| Pathway : | Antigen processing and presentation; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; KEGG pathway: Natural killer cell mediated cytotoxicity; Regulation of autophagy; Toll-like receptor signaling pathway. |
| Function : | interferon-alpha/beta receptor binding |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All IFNA2 Products
Download Datasheet