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| Cat. No.: | IFNA2-02H |
| Product Overview: | Recombinant Human Interferon alpha-2a produced inE. coliis a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19376 Dalton. Pegylated IFN alpha-2a is manufactured by attaching a 40KD NHS-mPEG to the primary amino acid of IFN alpha-2a. |
| Description: | At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end. |
| Purity: | >98% as determined by HPLC, FPLC and SDS-PAGE analysis. |
| Formulation: | Lyophilized from a (1mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer. |
| Specific Activity: | PEG-IFNα2a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0 x 106IU/mg. |
| Endotoxin: | Less than 0.1 ng/µg (1IEU/µg) of PEG-IFN alpha-2a. |
| Reconstitution: | It is recommended to reconstitute the lyophilized PEG-IFN alpha-2a in sterile 18M.-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage: | Lyophilized PEG-IFN alpha-2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PEG-IFN alpha-2a should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Gene Name: | IFNA2 |
| Synonyms: | IFNA; INFA2; MGC125764; MGC125765; Leukocyte interferon; B cell interferon, Type I interferon; IFNA2; IFN-α |
| Gene ID: | 3440 |
| mRNA Refseq: | NM_000605 |
| Protein Refseq: | NP_000596 |
| MIM: | 147562 |
| UniProt ID: | P01563 |
| Chromosome Location: | 9p22 |
| Pathway: | Antigen processing and presentation; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; KEGG pathway: Natural killer cell mediated cytotoxicity; Regulation of autophagy; Toll-like receptor signaling pathway. |
| Function: | interferon-alpha/beta receptor binding |
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