Active Recombinant Rat Vegfa

• Cat.No.: Vegfa-762R
• Product Overview: Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each.

Specification

• Description: Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor, is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties. During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188 which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR or to the non-tyrosine kinase neuropilin receptors NRP1/2.
• Source: P. pastoris
• Species: Rat
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50<4 ng/ml, measured by cell proliferation assay of>
• Molecular Mass: 38 kDa, observed by non-reducing SDS-PAGE.
• AA Sequence: Ala27-Arg190, expressed with an N-terminal Met.
• Endotoxin: <1 eu/μg, determined by lal>
• Purity: >95% as analyzed by reducing SDS-PAGE.
• Storage: Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrVEGF-A164 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164
• Gene ID: 83785
• mRNA Refseq: NM_001110334
• Protein Refseq: NP_001103804
• UniProt ID: P16612
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Hemostasis, organism-specific biosystem; Hypertrophy Model, organism-specific biosystem
• Function: cell surface binding; cell surface binding; chemoattractant activity; chemoattractant activity; cytokine activity; cytokine activity; fibronectin binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin binding; platelet-derived growth factor receptor binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding; vascular endothelial growth factor receptor binding

Reviews

04/21/2021

VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

03/09/2020

VEGFA they produced was highly active and the experimental effect was obvious.

Q&As

How does VEGFA protein relate to other biomarkers?

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment?

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis?

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs?

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy?

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease?

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.