Recombinant Canine VEGFA protein(Met1-Arg190)

• Cat.No.: VEGFA-959C
• Product Overview: Recombinant Canine VEGFA(NP_001103972.1) (Met1-Arg190) was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Non
• Protein Length: Met1-Arg190
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized canine VEGF164 / VEGFA at 10 μg/ml (100 μl/well) can bind human VEGFR2-Fc, The EC50 of human VEGFR2-Fc is 33.83-78.95 ng/ml. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 2-12 ng/mL.
• Molecular Mass: The recombinant canine VEGF164 / VEGFA comprises 164 amino acids and has a predicted molecular mass of 19.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Canis lupus familiaris (dog) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor 188
• Gene ID: 403802
• mRNA Refseq: NM_001003175
• Protein Refseq: NP_001003175
• UniProt ID: Q9MYV3