Recombinant Mouse Nt5e protein(Met1-Lys549), His-tagged

• Cat.No.: Nt5e-2302M
• Product Overview: Recombinant Mouse NT5E (NP_035981.1) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Lys549
• Form: Lyophilized from 20 mM Tris, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >10, 000 pmol/min/μg.
• Molecular Mass: The secreted recombinant mouse NT5E consists of 532 amino acids and has a calculated molecular mass of 59.4 kDa which is also detected in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nt5e 5 nucleotidase, ecto [ Mus musculus ]
• Official Symbol: Nt5e
• Synonyms: NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981