||TIMP metallopeptidase inhibitor 2, a tissue inhibitor of metalloproteinases, also known as TIMP2, is a human gene, thought to be a metastasis suppressor. This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells.
||The biological activity is measured by the ability to inhibit human MMP-2 hydrolysis of a fluorogenic MMP substrate.
||TIMP-2 has a greater binding efficiency to MMP-2 than the other MMPs. Although TIMP-2 is an inhibitor of MMP-2, it is also required at low concentrations for the activation of MMP-2.
||Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, 50 mM sodium chloride, and 0.05% sodium azide, pH 7.5.
||apparent mol wt ~20 kDa.