Recombinant Human SIRT3 Protein, His-tagged

• Cat.No.: SIRT3-112H
• Product Overview: Active recombinant N-terminal His-tagged human SIRT3 protein (101-399) was purified from E. coli.

Specification

• Description: SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 37 kDa (theoretical); 33.5 kDa (observed). The identity of SIRT3 protein was confirmed by mass spectrometry.
• Protein length: 101-399
• Purity: ≥60% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmole of 7-amino-4-methylcoumarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, containing 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 125 μM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(e-acetyl)-AMC), and 6mM NAD+.
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens (human) ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin 3; SIR2L3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin type 3; EC 2.3.1.286
• Gene ID: 23410
• mRNA Refseq: NM_012239
• Protein Refseq: NP_036371
• MIM: 604481
• UniProt ID: Q9NTG7