Recombinant Mouse Cul1 Protein, Myc/DDK-tagged

• Cat.No.: Cul1-2374M
• Product Overview: Purified recombinant protein of mouse full-length cullin 1 (Cul1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 90.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cul1 cullin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cul1
• Synonyms: CUL1; cullin 1; cullin-1; CUL-1
• Gene ID: 26965
• mRNA Refseq: NM_012042
• Protein Refseq: NP_036172
• UniProt ID: Q9WTX6

Reviews

06/16/2021

I highly recommend the CUL1 protein for a variety of research applications.

05/08/2016

It demonstrates excellent performance in ELISA, consistently delivering accurate and reliable results.

04/05/2016

the CUL1 protein has proven to be an invaluable tool in protein electron microscopy structure analysis.

Q&As

What techniques are used to study the expression and function of CUL1 protein in clinical research?

Techniques such as immunohistochemistry, Western blotting, and gene expression analysis are commonly used to study CUL1 protein expression and function in clinical research.

Is CUL1 protein involved in any other diseases besides cancer?

Yes, CUL1 protein has been implicated in various diseases, including neurodegenerative disorders and cardiovascular diseases, due to its role in protein degradation and cellular regulation.

Can CUL1 protein be used as a biomarker for cancer diagnosis?

While CUL1 protein levels are altered in certain cancers, it is not commonly used as a standalone biomarker. However, it may be part of a panel of markers used for cancer diagnosis.

Are there any genetic disorders associated with CUL1 protein mutations?

Yes, mutations in the CUL1 gene have been linked to a rare genetic disorder called Cullin-Ring Ligase 1 Syndrome, which is characterized by developmental delays and intellectual disabilities.

How does the dysregulation of CUL1 protein impact cell cycle progression?

Dysregulation of CUL1 protein can lead to the abnormal degradation of cell cycle regulators, resulting in uncontrolled cell division and potentially contributing to tumorigenesis.