| Description : |
Fibroblast growth factor (FGF) superfamily consists of 23 members, all of which contain a conserved 120 amino acid (aa) core region that contains six identical, interspersed amino acids. Based on the pI factor, two main groups of FGF are named: acidic FGF (aFGF) and basic FGF (bFGF). The superfamily members act extracellularly through four tyrosine kinase FGF receptors (FGR1 through FGR4), with multiple specificities noted for almost all FGFs. Biological binding of aFGF to its receptors will result in phosphorylation of the receptor and non-receptor proteins and generate its function. FGR2 (FGFR-2, Fibroblast growth factor receptor 2, Keratinocyte growth factor receptor 2, EC 2.7.1.112) is expressed mainly in bone, central nervous system, smooth muscle and tumors. Inhibition of FGR2 may inhibit cranial development and arterial repair as well as facilitate formation and proliferation of some tumors. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
456-770 aa |
| Form : |
0.926mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
6390U/mg, where one unit of FGFR2 (N549H), active activity is defined as 1nmol phosphate incorporated into 500μM (GGEEEEYFELVKKKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
38.1 kDa |
| AA Sequence : |
1 MHHHHHHEFK GLRRQDTPML AGVSEYELPE DPKWEFPRDK LTLGKPLGEG CFGQVVMAEA 61 VGIDKDKPKE AVTVAVKMLK DDATEKDLSD LVSEMEMMKM IGKHKNIINL LGACTQDGPL 121 YVIVEYASKG NLREYLRARR PPGMEYSYDI NRVPEEQMTF KDLVSCTYQL ARGMEYLASQ 181 KCIHRDLAAR NVLVTENNVM KIADFGLARD INNIDYYKKT TNGRLPVKWM APEALFDRVY 241 THQSDVWSFG VLMWEIFTLG GSPYPGIPVE ELFKLLKEGH RMDKPANCTN ELYMMMRDCW 301 HAVPSQRPTF KQLVEDLDRI LTLTTNEEYL |
| Purity : |
Purity 79.6% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| References : |
Kan S et al. (2002) Am.J.Hum.Genet;70:472-486) and Lajeunie E et al. (2006) European J Hum Genet; 14:289-298 |
