Active Recombinant Human IRAK1(194-end) Protein, N-His-tagged

• Cat.No.: IRAK1-111H
• Product Overview: N-terminal 6Histagged, recombinant, human IRAK1 amino acids 194–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: IRAK (IL-1 Receptor Associated Kinase 1) is a Ser/Thr kinase which associates with the IL-1 Receptor subunits after IL-1 binding, by virtue of the adapter protein MyD88. Three IRAK molecules, IRAK1, IRAK2, and IRAK-M, can relay the NF-&kB-activating signal via TNFR-associated factor 6 (TRAF6) and TGF-b-activated kinase 1 to the IKK complex, which ultimately mediates NF-&kB activation. Inhibition of IRAK1 may reduce inflammation as well as apoptosis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 194-end
• Form: 0.786mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 478U/mg, where one unit of IRAK1, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF RPSPFCWPLC EISRGTHNFS EELKIGEGGF 61 GCVYRAVMRN TVYAVKRLKE NADLEWTAVK QSFLTEVEQL SRFRHPNIVD FAGYCAQNGF 121 YCLVYGFLPN GSLEDRLHCQ TQACPPLSWP QRLDILLGTA RAIQFLHQDS PSLIHGDIKS 181 SNVLLDERLT PKLGDFGLAR FSRFAGSSPS QSSMVARTQT VRGTLAYLPE EYIKTGRLAV 241 DTDTFSFGVV VLETLAGQRA VKTHGARTKY LKDLVEEEAE EAGVALRSTQ STLQAGLAAD 301 AWAAPIAMQI YKKHLDPRPG PCPPELGLGL GQLACCCLHR RAKRRPPMTQ VYERLEKLQA 361 VVAGVPGHLE AASCIPPSPQ ENSYVSSTGR AHSGAAPWQP LAAPSGASAQ AAEQLQRGPN 421 QPVESDESLG GLSAALRSWH LTPSCPLDPA PLREAGCPQG DTAGESSWGS GPGSRPTAVE 481 GLALGSSASS SSEPPQIIIN PARQKMVQKL ALYEDGALDS LQLLSSSSLP GLGLEQDRQG 541 PEESDEFQS
• Purity: Purity 65.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: IRAK1 interleukin 1 receptor associated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: IRAK1
• Synonyms: IRAK1; interleukin 1 receptor associated kinase 1; IRAK; pelle
• Gene ID: 3654
• mRNA Refseq: NM_001569.4
• Protein Refseq: NP_001560.2
• MIM: 300283
• UniProt ID: P51617