Recombinant Human Interleukin-1 Receptor-Associated Kinase 1, GST-tagged

• Cat.No.: IRAK1-5436H
• Product Overview: Recombinant humanIRAK1 catalytic domain (amino acids 194-712), GST-tagged. No special measureswere taken to activate this kinase.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 194-712 a.a.
• Description: Nuclear factor kappa B (NF-κB)is a ubiquitous transcription factor and an essential mediator of geneexpression during activation of immune and inflammatory responses. NF-κBmediates the expression of a great variety of genes in response toextracellular stimuli including IL-1, TNFα and LPS. A serine/threonineprotein kinase associated with IL-1 receptor (IRAK) and its homologue mousepelle-like protein kinase (mPLK) were identified recently. IRAK is associatedwith the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding andserves as a signaling molecule to mediate IL-1 response.
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mM DTT +50% glycerol.
• Purity: ≥ 95% as determinedby SDS-PAGE analysis
• Molecular Weight: 83.4 kDa
• Biological Activity: 255 nmoleof phosphate transferred to myelin basic protein (MBP) per minute per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 3.33μg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: IRAK1

Gene Information

• Gene Name: IRAK1 interleukin-1receptor-associated kinase 1 [ Homo sapiens ]
• Synonyms: IRAK1; interleukin-1receptor-associated kinase 1; IRAK; pelle; EC 2.7.11.1; interleukin-1receptor-associated kinase 1; Pelle homolog
• Gene ID: 3654
• mRNA Refseq: NM_001025242
• Protein Refseq: NP_001020413
• MIM: 300283
• UniProt ID: P51617
• Chromosome Location: Xq28
• Pathway: Apoptosis; Neurotrophinsignaling pathway; Toll-like receptor signaling pathway; Signaling in Immunesystem; Signalling by NGF
• Function: ATP binding; NF-kappaB-inducingkinase activity; interleukin-1 receptor binding; nucleotide binding; proteinhomodimerization activity; protein serine/threonine kinase activity;transcription activator activity; transferase activity