Recombinant Human IRAK1 293 Cell Lysate

• Cat.No.: IRAK1-5174HCL

Specification

• Description: Antigen standard for interleukin-1 receptor-associated kinase 1 (IRAK1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: IRAK1 interleukin-1 receptor-associated kinase 1 [ Homo sapiens ]
• Official Symbol: IRAK1
• Synonyms: IRAK1; interleukin-1 receptor-associated kinase 1; IRAK; pelle; IRAK-1; Pelle homolog
• Gene ID: 3654
• mRNA Refseq: NM_001025243
• Protein Refseq: NP_001020414
• MIM: 300283
• UniProt ID: P51617
• Chromosome Location: Xq28
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem
• Function: ATP binding; NF-kappaB-inducing kinase activity; interleukin-1 receptor binding; kinase activity; nucleotide binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein homodimerization activity; protein kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; ubiquitin-protein ligase activity

Reviews

04/01/2021

The IRAK1 protein stands out for its exceptional quality, making it an excellent choice to meet my experimental requirements.

04/21/2020

The IRAK1 protein's compatibility with various experimental techniques adds to its versatility.

05/12/2017

Its stability and compatibility make it an excellent tool for visualizing and characterizing protein structures at high resolution.

Q&As

Are there any ongoing clinical trials targeting IRAK1 for cancer treatment?

Yes, there are several clinical trials exploring the inhibition of IRAK1 as a therapeutic strategy for various types of cancer.

How is IRAK1 protein implicated in autoimmune diseases?

IRAK1 is involved in the signaling pathways that can lead to the development of autoimmune diseases by regulating the immune system's response to self-antigens.

How does the expression of IRAK1 relate to inflammatory diseases?

Elevated levels of IRAK1 expression have been observed in inflammatory diseases, suggesting its involvement in the dysregulation of inflammatory responses.

In what ways can IRAK1 be a target for drug development in inflammatory conditions?

Inhibiting IRAK1 activity is being investigated as a potential therapeutic approach for managing inflammatory conditions, offering a target for drug development.

What clinical significance does IRAK1 hold in cancer research?

IRAK1 has been identified as a potential target for cancer therapy, as it plays a role in promoting tumor growth and evasion of the immune system.