IRAK1

  • Official Full Name

    interleukin-1 receptor-associated kinase 1

  • Overview

    Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor. The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/I
  • Synonyms

    IRAK1; interleukin-1 receptor-associated kinase 1; IRAK; pelle; IRAK-1; Pelle homolog;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Human
  • Human
  • Mouse
  • E.coli
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Insect cell
  • Insect Cell
  • Sf21
  • Wheat Germ
  • GST
  • His
  • Myc
  • DDK
  • MYC
  • N/A
  • N
  • C
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human IRAK1-111H Active Recombinant Human IRAK1(194-end) Protein, N-His-tagged Sf21 His 194-end
Human IRAK1-1542H Recombinant Human IRAK1 Protein, His tagged E.coli N-His
Human IRAK1-5436H Recombinant Human Interleukin-1 Receptor-Associated Kinase 1, GST-tagged Insect Cell GST
Human IRAK1-7068H Recombinant Human IRAK1 protein, His-tagged E.coli His Phe212~Glu440 (Accession # P51617)
Human IRAK1-27500TH Recombinant Human IRAK1 Wheat Germ N/A 633 amino acids
Human IRAK1-27502TH Recombinant Human IRAK1 Wheat Germ N/A 164 amino acids
Human IRAK1-5173HCL Recombinant Human IRAK1 293 Cell Lysate HEK293 N/A
Human IRAK1-5174HCL Recombinant Human IRAK1 293 Cell Lysate HEK293 N/A
Human IRAK1-869H Recombinant Human IRAK1 protein, His-tagged Insect cell His
Human IRAK1-1200H Recombinant Human IRAK1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Human IRAK1-2103H Recombinant Human IRAK1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human IRAK1-5056H Recombinant Human IRAK1 Protein, GST-tagged Wheat Germ GST
Human IRAK1-1171H Recombinant Human IRAK1 Protein (R194-S712), GST tagged Insect Cell GST R194-S712
Human IRAK1-5726HF Recombinant Full Length Human IRAK1 Protein, GST-tagged In Vitro Cell Free System GST 633 amino acids
Human IRAK1-799H Recombinant Human IRAK1 Protein, GST/His-tagged E.coli N-GST and C-His
Human IRAK1-257HF Recombinant Full Length Human IRAK1 Protein In Vitro Cell Free System 633 amino acids
Human IRAK1-01H Active Recombinant Human IRAK1 Protein, GST-tagged Insect Cell GST
Human IRAK1-1170H Recombinant Human IRAK1 Protein (R194-S712), Tag Free Insect Cell No tag R194-S712
Human IRAK1-2647H Recombinant Human IRAK1 Protein (Phe212-Leu434), N-His tagged E.coli N-His Phe212-Leu434
Mouse Irak1-1243M Recombinant Mouse Irak1 Protein, MYC/DDK-tagged HEK293T MYC/DDK
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • IRAK1 Related Signal Pathway

What is IRAK1 protein?

The Interleukin-1 Receptor-Associated Kinase 1, also known as IRAK1 protein, is a critical player in the innate immunity and a key component of the signaling pathway of the interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs).

In the quest to gain a thorough knowledge of mammalian Toll-like receptor signaling, IRAK1 was discovered by the Cao and Dixon research team in 1996. It was identified as a IL-1R-associated protein kinase that plays a crucial role in normal immune responses. This finding bridged the gap in understanding the molecular mechanisms underpinning pathogen recognition and the subsequent immune response.

Gene Locus and Protein Structure

IRAK1 gene is located on the X Chromosome's long arm at the position Xq28, an area notorious for containing sequences implicated in debilitating genetic disorders such as Hemophilia A and B, and G6PD deficiency.

The IRAK1 protein is composed of 712 amino acids and has a molecular mass of about 78kDa. It is structurally composed of a central kinase domain, an N-terminal Death Domain (DD), and a C-terminal domain. The DD is used for interactions with MyD88, an adapter protein, facilitating downstream signaling events.

Functions of IRAK1 Protein

One of the primary roles of the IRAK1 protein is its involvement in immunity-related signal transduction pathways. Upon the recognition of microbial components by TLRs or IL-1R, IRAK1 is recruited to the receptor complexes, leading to its activation. This activation initiates a signal transduction cascade resulting in the activation of certain transcription factors like NF-κB and AP-1, which in turn produce a range of pro-inflammatory cytokines.

Additionally, IRAK1 protein also influences adaptive immunity. Recent studies reveal that IRAK1 indirectly modulates adaptive immune system by steering the polarization of helper T cells.

IRAK1 Protein Related Signal Pathway

IRAK1 participates in the TLR and IL-1R signaling pathways. Following a pathogen's invasion, TLRs and IL-1R recognize distinct microbial components, leading to the recruitment of the adapter molecule MyD88. MyD88 binds to IRAK4 and IRAK1, causing the activation of IRAK1. Activated IRAK1 then dissociates from the receptor complex and associates with the E3 ubiquitin ligase TRAF6, leading to activation of downstream molecules like MAPK and NF-kB, which catalyze the production of pro-inflammatory cytokines.

IRAK1 Protein Related Diseases

Mutations or dysregulations in IRAK1 signaling have been strongly linked to a multitude of diseases, primarily autoimmune and inflammatory diseases. The abnormal activation of IRAK1 has also been found in a variety of cancers including breast, gastric, and lung cancers, indicating a potentially significant role in tumorigenesis.

IRAK1 has been implicated in the pathogenesis of systemic lupus erythematosus (SLE). In SLE patients, a higher level of IRAK1 was observed, leading to inflammation and autoantibody production. IRAK1 has also been recognized as a potential contributor to Alzheimer's disease, as aberrant IRAK1 activity leads to chronic inflammation, a likely factor in Alzheimer's' disease progression.

  •  Inflammatory disorders: IRAK1 plays a crucial role in the activation of inflammatory pathways, such as the Toll-like receptor (TLR) signaling pathway. Abnormal IRAK1 activation or expression has been implicated in the pathogenesis of inflammatory disorders, including rheumatoid arthritis, systemic lupus erythematosus, and inflammatory bowel disease.
  •  Infectious diseases: The TLR signaling pathway, in which IRAK1 is involved, is critical in the immune response against pathogens. Dysregulation of IRAK1 signaling can contribute to susceptibility to various infections, including bacterial, viral, and fungal infections.
  •  Cancer: IRAK1 has been linked to the development and progression of several types of cancer. Aberrant IRAK1 activation promotes tumor growth, survival, and invasion by modulating various signaling pathways involved in cell proliferation, apoptosis, and inflammation. IRAK1 overexpression or hyperactivation has been observed in cancers such as breast cancer, lung cancer, colorectal cancer, and pancreatic cancer.

IRAK1 Protein's Applications

Considering the central role of IRAK1 in immune responses and its connection to various diseases, it's regarded as a promising therapeutic target for pharmaceutical interventions. Small-molecule inhibitors targeting IRAK1, such as the FDA-approved drug pacritinib, have shown encouraging results, especially in treating certain types of leukemia.

High Purity

SDS-PAGE IRAK1-1542H.jpg

Fig1. SDS-PAGE (Cat. No.: IRAK1-1542H)

High Bioactivity & Detection Sensitivity

IRAK1 bioactivity.jpg

Fig2. The specific activity of IRAK1 was determined to be 31 nmol/min/mg

Characterization of the Differentially Methylated Region in IRAK1.jpg

(Undraga Schagdarsurengin, 2022)

Fig3. Characterization of the Differentially Methylated Region in IRAK1 (IRAK1-DMR). (A) A snapshot of graphical user interface from ChAMP (Chip Analysis Methylation Pipeline) package showing a trend of DNA methylation levels (β-values) at different CpG-sites (presented as CpG-probes cg-number) in normal prostate (NOR) and prostate cancer tissue (TU). Lines represent mean methylation levels of analyzed CpG-probes (NOR, blue; TU, yellow). (B) Correlation plots of IRAK1-mRNA levels and IRAK1-methylation values in different CpG-probes (Spearman's correlation coefficient r is indicated for each correlation analysis).

High expression of IRAK1 in HCC tissues.jpg

(Ning Li, 2016)

Fig4. High expression of IRAK1 in HCC tissues (T) comparing with adjacent normal tissues (N). c Western Blot analysis of IRAK1 in HCC tumor tissues (T1-12) and adjacent liver tissues (N1-12).

Variation of IRAK1 expression and overall survival.jpg

(Chaoying Song, 2022)

Fig5. Variation of IRAK1 expression and overall survival of hepatocellular carcinoma based on pathology TNM stages and radiation therapy status. (a) IRAK1 vs. T stage (p = 0.1675, Kruskal-Wallis test); (b) IRAK1 vs. N stage (p = 0.2957, Wilcox test); (c) IRAK1 vs. M stage (p = 0.6619, Wilcox test); (d) IRAK1 vs. radiation therapy (p = 0.3961, Wilcox test).

IRAK1 involved in several pathways and played different roles in them. We selected most pathways IRAK1 participated on our site, such as NF-kappa B signaling pathway, Apoptosis, Toll-like receptor signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with IRAK1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
NF-kappa B signaling pathwayGM10591;CD40;PLCG2;TNFSF14;BCL10;TRAF5;BCL2L1;RELB;ERC1
ApoptosisBCAP31;CDK5RAP2;CFLAR;CAPN1B;MYC;DIABLOB;TNF;CASP7;CAPN2L
Toll-like receptor signaling pathwayNFKBIAA;TLR2;IFNA16;TLR4BB;LBP;PIK3CD;MAPK14B;IFNA12;CCL4
Neurotrophin signaling pathwayPIK3R2;CALM3;RHOA;Fasl;KIDINS220;MAPK9;YWHAE;MAPK3;NFKBIE
PertussisCXCL5;MYD88;MAPK8;C1S;CXCL6;NFKB1;IRF8;CALM3;RHOA
LeishmaniasisMAP3K7;FCGR3B;HLA-DPA1;ITGAM;NCF2;MYD88;Itgam&Itgb2;IFNG;HLA-DOB
Chagas disease (American trypanosomiasis)IKBKB;TGFB1;CASP8;IFNG;GNA11;CD3D;CD3E; CD3D;AKT1;C1QC
ToxoplasmosisHSPA1B;Toxoplasma Gondii Dense Granule Protein 7;ITGB1;TNF;IKBKG;ALOX5;IFNGR2;PDPK1;LDLR
TuberculosisIL1B;MAPK1;IFNA6;IFNA17;ARHGEF12;CALML5;RFX5;MYD88;ATP6V0A1
MeaslesIL12A;EIF3H;CD46;TP53;MAVS;TRAF6;CD209C;TLR9;FAS
Epstein-Barr virus infectionHLA-A;PSMD6;MAP3K7;POLR3H;MAPK8;POLR2J2;SPI1;NFKB2;CDK1

IRAK1 has several biochemical functions, for example, ATP binding, NF-kappaB-inducing kinase activity, heat shock protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by IRAK1 itself. We selected most functions IRAK1 had, and list some proteins which have the same functions with IRAK1. You can find most of the proteins on our site.

Function Related Protein
ATP bindingIGHMBP2;NTPCR;ABCF1;PIM3;IDE;GLUL;ETNK2;MYH4;DDX23
NF-kappaB-inducing kinase activityIRAK1;ALPK2;MAP3K14;RIPK3;ALK;IKBKE;PPP4C
heat shock protein bindingDMPK;PPP5C;NFKB1;HSPA1A;DNAJA3A;GRXCR2;APAF1;SSUH2.2;FKBP4
interleukin-1 receptor bindingIL1A;IL1F10;IL36G;Il1f6;IL1RAP;IL36RN;IL1RN;IL37;TLR9
kinase activityWNK2;HMGXB3;PKN1B;CAMK1GB;BSK146;GM4922;TGFBR1B;PRPS2;STK38A
protein bindingNFE2L2;GOLPH3L;OTULIN;ZNF394;ASAP1;KIAA0146;ADM;NTF4;NSFL1C
protein heterodimerization activityGTF2A1;SUPT4H1;KCNH1;HIST1H2AB;P2RY1;TCF3B;TAS1R2;SUPT5H;CD3G
protein homodimerization activityARID3A;PCYT1A;ACHE;PVRL1A;Trl;GYS2;BMPR1A;HMGCS1;ZBTB1
protein kinase activityRAF1A;ACVR1BA;CAMK1DA;PLK1;STK25;IRAK4;STK26;PRKAG1;RPS6KB1B
protein serine/threonine kinase activityNEK8;STK32B;DSTYK;MAPK12;HIPK2;PKN1B;EIF2AK2;MAP4K3;PIM2
ubiquitin-protein transferase activityBRAP;MIB1;KLHL20;ARIH1;FBXL5;FBXO25;CDC34;RNF144B;UBE2O

IRAK1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with IRAK1 here. Most of them are supplied by our site. Hope this information will be useful for your research of IRAK1.

TRAF6; PELI2; PIN1; MYD88; PELI1

Research Area

Related articles

Zhang, JM; Jia, G; et al. Silencing miR-146a influences B cells and ameliorates experimental autoimmune myasthenia gravis. IMMUNOLOGY 144:56-67(2015).
Ciccacci, C; Perricone, C; et al. A Multilocus Genetic Study in a Cohort of Italian SLE Patients Confirms the Association with STAT4 Gene and Describes a New Association with HCP5 Gene. PLOS ONE 9:-(2014).
  • Q&As
  • Reviews

Q&As (5)

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Are there any ongoing clinical trials targeting IRAK1 for cancer treatment? 03/16/2023

Yes, there are several clinical trials exploring the inhibition of IRAK1 as a therapeutic strategy for various types of cancer.

How is IRAK1 protein implicated in autoimmune diseases? 06/19/2020

IRAK1 is involved in the signaling pathways that can lead to the development of autoimmune diseases by regulating the immune system's response to self-antigens.

How does the expression of IRAK1 relate to inflammatory diseases? 01/08/2020

Elevated levels of IRAK1 expression have been observed in inflammatory diseases, suggesting its involvement in the dysregulation of inflammatory responses.

In what ways can IRAK1 be a target for drug development in inflammatory conditions? 08/23/2016

Inhibiting IRAK1 activity is being investigated as a potential therapeutic approach for managing inflammatory conditions, offering a target for drug development.

What clinical significance does IRAK1 hold in cancer research? 03/31/2016

IRAK1 has been identified as a potential target for cancer therapy, as it plays a role in promoting tumor growth and evasion of the immune system.

Customer Reviews (3)

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Reviews
04/01/2021

    The IRAK1 protein stands out for its exceptional quality, making it an excellent choice to meet my experimental requirements.

    04/21/2020

      The IRAK1 protein's compatibility with various experimental techniques adds to its versatility.

      05/12/2017

        Its stability and compatibility make it an excellent tool for visualizing and characterizing protein structures at high resolution.

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