Active Recombinant Human MYO3B(1-326) Protein, N-GST-tagged

• Cat.No.: MYO3B-169H
• Product Overview: N-terminal GST-tagged, recombinant human MYO3B, amino acids 1-326 expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with MYO3B include Deafness, Autosomal Recessive 30 and Entropion. Among its related pathways are Sensory processing of sound and PAK Pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MYO3A.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-326 aa
• Form: 1.82 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 513 U/mg, where one unit of MYO3B activity is defined as 1 nmol phosphate incorporated into 250 μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 64 kDa
• Purity: 99% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MYO3B myosin IIIB [ Homo sapiens (human) ]
• Official Symbol: MYO3B
• Synonyms: MYO3B; myosin IIIB; Myosin IIIB; Myosin-IIIb; EC 2.7.11.1
• Gene ID: 140469
• mRNA Refseq: NM_138995.5
• Protein Refseq: NP_620482.3
• MIM: 610040
• UniProt ID: Q8WXR4