| Description : |
Three tyrosine kinase receptors, designated TRKA, TRKB and TRKC, have been identified that selectively bind NGF, BDNF and NT-3, respectively; NT-4/5 also binds to the TRKB receptor. NGF binds to their specific TRK receptor to stimulate intracellular signaling pathways leading to specific protein phosphorylations and differential gene inductions. TRKA (High affinity nerve growth factor, TRK1 transforming tyrosine kinase protein, p140-TrkA, Trk-A) receptor kinases engage the activation of a branched proto-oncoprotein signaling pathway involving Shc, Src, Ras, Raf, mitogen-activated protein kinase, and other downstream effectors. Inhibitors of TRKA may be useful in the treatment of cancer. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
440-end |
| Form : |
0.768mg/ml of enzyme in 50mM Tris/HCl pH8.5 (at 4 centigrade), 300mM NaCl, 5% glycerol, 0.1mM EGTA, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
265U/mg, where one unit of TRKA, active activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
41 kDa |
| AA Sequence : |
1 MHHHHHHEFN KCGRRNKFGI NRPAVLAPED GLAMSLHFMT LGGSSLSPTE GKGSGLQGHI 61 IENPQYFSDA CVHHIKRRDI VLKWELGEGA FGKVFLAECH NLLPEQDKML VAVKALKEAS 121 ESARQDFQRE AELLTMLQHQ HIVRFFGVCT EGRPLLMVFE YMRHGDLNRF LRSHGPDAKL 181 LAGGEDVAPG PLGLGQLLAV ASQVAAGMVY LAGLHFVHRD LATRNCLVGQ GLVVKIGDFG 241 MSRDIYSTDY YRVGGRTMLP IRWMPPESIL YRKFTTESDV WSFGVVLWEI FTYGKQPWYQ 301 LSNTEAIDCI TQGRELERPR ACPPEVYAIM RGCWQREPQQ RHSIKDVHAR LQALAQAPPV 361 YLDVLG |
| Purity : |
Purity 67% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
