Active Recombinant Human NTRK1 protein, His-hFc-tagged

• Cat.No.: NTRK1-155H
• Product Overview: Active Recombinant Human NTRK1 protein(NP_002520.2)(Met 1-Pro 382) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-382 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.04-0.15 μg/ml in the presence of 10 ng/ml of recombinant human NGF.
• Molecular Mass: The recombinant human NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 598 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NTRK1/Fc monomer is approximately 85-95 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001007793.1
• Unit ID: P04629
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P04629

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; MTC; TRK; TRKA; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; TRK1; Trk-A; p140-TrkA; DKFZp781I14186
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• protein Refseq: NP_001007793
• MIM: 191315