Source : |
HEK293 |
Species : |
Human |
Tag : |
Fc |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : |
Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1 Protein, Fc Tag with a linear range of 0.1-3 ng/mL. Immobilized Human PD-1 Protein, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL. Immobilized Biotinylated Human PD-L2, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human PD-1 Protein, Fc Tag with a linear range of 0.1-1 ng/mL.Serial dilutions of nivolumab were added into Human PD-1 Protein, Fc Tag: Biotinylated Human PD-L1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 42.1 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Leu25-Gln167 |
Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |