Active Recombinant Human PDCD1 Protein, Fc-Avi-His-tagged, Biotinylated

• Cat.No.: PDCD1-258H
• Product Overview: Biotinylated Human PD-1 / PDCD1, His Tag & Fc Tag is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_005009). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc&His
• Protein Length: 25-167 a.a.
• Predicted N Terminal: Leu 25
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 ul/well),can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 ug/mL. Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, His Tag, Fc tag can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1ug/mL. FACS analysis shows that the binding of Biotinylated Human PD-1, His Tag, Fc tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD1 used is 1ug/mL. The IC50 is 0.362 ug/mL.
• Molecular Mass: The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation and 100-130 kDa under non-reducing condition.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116