Active Recombinant Human PDCD1 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: PDCD1-260H
• Product Overview: Biotinylated Human PD-1, Fc Tag is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # Q15116-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 25-167 a.a.
• Predicted N Terminal: Leu 25
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc Tag with a linear range of 20-320 ug/mL.
• Molecular Mass: The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-66 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116