Active Recombinant Human STK25(1-308) Protein, N-GST,C-His-tagged

• Cat.No.: STK25-234H
• Product Overview: N-terminal GST-tagged and C-terminal 6His-tagged, recombinant, human STK25 amino acids 1-308, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.

Specification

• Description: STK25 is a member of the germinal center kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-308 aa
• Form: 0.961mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 382U/mg, where one unit of STK25, active activity is defined as 1nmol phosphate incorporated into 250μM (RLGRDKYKTLRQIRQ) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MAHLRGFANQ 241 HSRVDPEELF TKLDRIGKGS FGEVYKGIDN HTKEVVAIKI IDLEEAEDEI EDIQQEITVL 301 SQCDSPYITR YFGSYLKSTK LWIIMEYLGG GSALDLLKPG PLEETYIATI LREILKGLDY 361 LHSERKIHRD IKAANVLLSE QGDVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPEVIKQS 421 AYDFKADIWS LGITAIELAK GEPPNSDLHP MRVLFLIPKN SPPTLEGQHS KPFKEFVEAC 481 LNKDPRFRPT AKELLKHKFI TRYTKKTSFL TELIDRYKRW KSEGHGEESS SEDSDIDGHH 541 HHHH
• Purity: Purity 94% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK25 serine/threonine kinase 25 [ Homo sapiens (human) ]
• Official Symbol: STK25
• Synonyms: STK25; serine/threonine kinase 25; SOK1; YSK1
• Gene ID: 10494
• mRNA Refseq: NM_001271977.2
• Protein Refseq: NP_001258906.1
• MIM: 602255
• UniProt ID: O00506