Active Recombinant Human STK35(130-end) Protein, N-His-tagged

• Cat.No.: STK35-50H
• Product Overview: N-terminal 6Histagged, recombinant, human CLK1, amino acids 130-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CLK1 is a dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 130-end
• Form: 0.445mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 848U/mg, where one unit of CLK1, active activity is defined as 1nmol phosphate incorporated into 200μM (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 45 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GKSHRRKRTR SVEDDEEGHL ICQSGDVLSA 61 RYEIVDTLGE GAFGKVVECI DHKAGGRHVA VKIVKNVDRY CEAARSEIQV LEHLNTTDPN 121 STFRCVQMLE WFEHHGHICI VFELLGLSTY DFIKENGFLP FRLDHIRKMA YQICKSVNFL 181 HSNKLTHTDL KPENILFVQS DYTEAYNPKI KRDERTLINP DIKVVDFGSA TYDDEHHSTL 241 VSTRHYRAPE VILALGWSQP CDVWSIGCIL IEYYLGFTVF PTHDSKEHLA MMERILGPLP 301 KHMIQKTRKR KYFHHDRLDW DEHSSAGRYV SRRCKPLKEF MLSQDVEHER LFDLIQKMLE 361 YDPAKRITLR EALKHPFFDL LKKSI
• Purity: Purity 86% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK35 serine/threonine kinase 35 [ Homo sapiens (human) ]
• Official Symbol: STK35
• Synonyms: STK35; serine/threonine kinase 35; CLIK1; STK35L1
• Gene ID: 140901
• mRNA Refseq: NM_080836.4
• Protein Refseq: NP_543026.2
• MIM: 609370
• UniProt ID: Q8TDR2