Recombinant Human EBI3&IL12A protein(Met1-Lys229 & Arg23-Ser219), hFc-tagged

• Cat.No.: EBI3&IL12A-1270H
• Product Overview: Recombinant Human IL35 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of Human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Lys229 & Arg23-Ser219
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Human IL35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL35/Fc monomer is approximately 90-100 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EBI3 Epstein-Barr virus induced 3 [ Homo sapiens ]
• Official Symbol: EBI3; IL12A
• Synonyms: EBI3; Epstein-Barr virus induced 3; interleukin-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; IL-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; IL27B; IL-27B
• Gene ID: 10148
• mRNA Refseq: NM_005755
• Protein Refseq: NP_005746
• MIM: 605816
• UniProt ID: Q14213