Active Recombinant Full Length Human STK35 Protein, N-His-tagged

• Cat.No.: STK35-59HFL
• Product Overview: N-Terminal 6His-tagged, recombinant, human CLIK1 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.59 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 66 U/mg, where one unit of CLIK1 activity is defined as 1 nmol phosphate incorporated into 300 μM RSRSRSRSRSRSRSR per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 49 kDa
• Purity: 59% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK35 serine/threonine kinase 35 [ Homo sapiens (human) ]
• Official Symbol: STK35
• Synonyms: STK35; serine/threonine kinase 35; CLIK1; STK35L1
• Gene ID: 140901
• mRNA Refseq: NM_080836.4
• Protein Refseq: NP_543026.2
• MIM: 609370
• UniProt ID: Q8TDR2