Active Recombinant Full Length Human TP53 Protein, N-cMyc,GST-tagged

• Cat.No.: TP53-123HFL
• Product Overview: Nterminal cMyc and GSTtagged, recombinant, human p53 full length expressed in E coli. Purified using glutathione agarose and gel filtration.

Specification

• Description: p53 is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
• Source: E. coli
• Species: Human
• Tag: cMyc,GST
• Protein length: Full Length
• Form: 0.34mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Mass: 72 kDa
• AA Sequence: 1 MEQKLISEED LSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL 61 EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA 121 YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL 181 DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM 241 EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE QWFTEDPGPD 301 EAPRMPEAAP RVAPAPAAPT PAAPAPAPSW PLSSSVPSQK TYQGSYGFRL GFLHSGTAKS 361 VTCTYSPALN KMFCQLAKTC PVQLWVDSTP PPGTRVRAMA IYKQSQHMTE VVRRCPHHER 421 CSDSDGLAPP QHLIRVEGNL RVEYLDDRNT FRHSVVVPYE PPEVGSDCTT IHYNYMCNSS 481 CMGGMNRRPI LTIITLEDSS GNLLGRNSFE VRVCACAGRD RRTEEENLRK KGEPHHELPP 541 GSTKRALPNN TSSSPQPKKK PLDGEYFTLQ IRGRERFEMF RELNEALELK DAQAGKEPGG 601 SRAHSSHLKS KKGQSTSRHK KLMFKTEGPD SD
• Purity: Purity 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens (human) ]
• Official Symbol: TP53
• Synonyms: TP53; tumor protein p53; P53; BCC7; LFS1; BMFS5; TRP53
• Gene ID: 7157
• mRNA Refseq: NM_000546.6
• Protein Refseq: NP_000537.3
• MIM: 191170
• UniProt ID: P04637