| Description : |
PTP-1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
1-321 aa |
| Form : |
0.637mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 10% glycerol, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
11537U/mg, where one unit of PTP-1B, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8- difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature. |
| Molecular Mass : |
64.1 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM EMEKEFEQID 241 KSGSWAAIYQ DIRHEASDFP CRVAKLPKNK NRNRYRDVSP FDHSRIKLHQ EDNDYINASL 301 IKMEEAQRSY ILTQGPLPNT CGHFWEMVWE QKSRGVVMLN RVMEKGSLKC AQYWPQKEEK 361 EMIFEDTNLK LTLISEDIKS YYTVRQLELE NLTTQETREI LHFHYTTWPD FGVPESPASF 421 LNFLFKVRES GSLSPEHGPV VVHCSAGIGR SGTFCLADTC LLLMDKRKDP SSVDIKKVLL 481 EMRKFRMGLI QTADQLRFSY LAVIEGAKFI MGDSSVQDQW KELSHEDLEP PPEHIPPPPR 541 PPKRILEPHN |
| Purity : |
Purity 46% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
