Active Recombinant Human PTPN7(22-end) Protein, N-His-tagged

• Cat.No.: PTPN7-99H
• Product Overview: N-terminal 6Histagged, recombinant, human HePTP, amino acids 22–end, expressed in E. coli cells. Purified using Ni2+/NTA agarose.

Specification

• Description: HePTP is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: 22-end
• Form: 0.812mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 10% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 8316U/mg, where one unit of HePTP, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 40.7 kDa
• AA Sequence: 1 MGSSHHHHHH SSGLVPRGSH MTQPPPEKTP AKKHVRLQER RGSNVALMLD VRSLGAVEPI 61 CSVNTPREVT LHFLRTAGHP LTRWALQRQP PSPKQLEEEF LKIPSNFVSP EDLDIPGHAS 121 KDRYKTILPN PQSRVCLGRA QSQEDGDYIN ANYIRGYDGK EKVYIATQGP MPNTVSDFWE 181 MVWQEEVSLI VMLTQLREGK EKCVHYWPTE EETYGPFQIR IQDMKECPEY TVRQLTIQYQ 241 EERRSVKHIL FSAWPDHQTP ESAGPLLRLV AEVEESPETA AHPGPIVVHC SAGIGRTGCF 301 IATRIGCQQL KARGEVDILG IVCQLRLDRG GMIQTAEQYQ FLHHTLALYA GQLPEEPSP
• Purity: Purity 72.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTPN7 protein tyrosine phosphatase non-receptor type 7 [ Homo sapiens (human) ]
• Official Symbol: PTPN7
• Synonyms: PTPN7; protein tyrosine phosphatase non-receptor type 7; LPTP; HEPTP; PTPNI; BPTP-4; LC-PTP
• Gene ID: 5778
• mRNA Refseq: NM_002832.4
• Protein Refseq: NP_002823.4
• MIM: 176889
• UniProt ID: P35236