"IDH3G" Related Products

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Recombinant Human Isocitrate Dehydrogenase 3 (NAD+) Gamma, His-tagged

Cat. No.: IDH3G-1324H
Product Overview: Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description: IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD+ as the electron acceptor, and is localised to the mitochondrial matrix.
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 5 mM DTT, 50% glycerol, 0.2 M NaCl, 2 mM EDTA.
Molecular Weight: 41.1 kDa (375 aa), confirmed by MALDI-TOF
Purity: > 85 % by SDS-PAGE
Concentration: 0.25 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MFSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Citrate cycle (TCA cycle); Citrate cycle; Citric acid cycle (TCA cycle); Metabolic pathways; Pyruvate metabolism and Citric Acid (TCA) cycle; TCA Cycle
Gene Name: IDH3G isocitrate dehydrogenase 3 (NAD+) gamma [ Homo sapiens ]
Official Symbol: IDH3G
Synonyms: IDH3G; isocitrate dehydrogenase 3 (NAD+) gamma; H-IDHG; isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; IDH-gamma; NAD+-specific ICDH; OTTHUMP00000025984; OTTHUMP00000025985; OTTHUMP00000025987; OTTHUMP00000025988; OTTHUMP00000214764; NAD+-specific ICDH subunit gamma; isocitric dehydrogenase subunit gamma; NAD (H)-specific isocitrate dehydrogenase gamma subunit; isocitrate dehydrogenase, NAD+-specific, mitochondrial, gamma subunit; NP_004126.1; EC 1.1.1.41
Gene ID: 3421
mRNA Refseq: NM_004135
Protein Refseq: NP_004126
MIM: 300089
UniProt ID: P51553
Chromosome Location: Xq28
Function: ATP binding; NAD binding; isocitrate dehydrogenase (NAD+) activity; magnesium ion binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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