Active Recombinant Mouse Saa1 Protein

• Cat.No.: Saa1-185M
• Product Overview: Recombinant Mouse Saa1(Gly20-Tyr122) was expressed in E. coli.

Specification

• Description: Mouse Serum Amyloid A protein‑1 (SAA1; previously SAA2 in mouse) is a multifunctional apolipoprotein produced by hepatocytes in response to pro‑inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated protein and circulates as part of the HDL complex. The SAA1 gene is one of five SAA genes in mouse. Mature mouse SAA1 shares 72%, 72% and 67% amino acid (aa) sequence identity with human, rabbit and equine SAA1, respectively. SAA1 and SAA2 share 113 of their 122 amino acids and are termed A‑SAA (acute phase SAA). SAA1 is produced in the liver in both human and mouse. SAA1 is prominently produced in human adipose tissue, but is absent in mouse adipose, which instead expresses adipose SAA3 (a pseudogene in humans). In mouse, however, circulating SAA1 is elevated by insulin resistance. A‑SAA can increase by as much as 1000‑fold during inflammation. When highly expressed, A‑SAA can displace ApoA1 as the major apolipoprotein in HDL complexes, weakening its role as a reverse (lipid clearing) cholesterol transporter. A highly charged region of SAA1 and 2 (aa 36 ‑ 68) contains putative fibronectin and laminin binding motifs. This region also binds heparan sulfate proteoglycans at mildly acidic pH and promotes aggregation of A‑SAA; however, pathogenic amyloid fibrils contain fragments of mouse SAA2, not SAA1. Mouse strains can differ in SAA sequence, expression, and amyloid formation. SAA1 is a ligand for CD36/SR‑B3, SR‑B1, FPRL1, TLR2, and RAGE on monocytes/macrophages, inducing chemotaxis and generation of cytokines and tissue factor. SAA1 can bind the surface of invading gram‑negative bacteria, acting as an opsonin to aid clearance by macrophages. SAA1 also binds platelets, probably by engaging fibrinogen on the platelet surface.
• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Gly20
• Form: Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl, PEG and Imidazole.
• Bio-activity: Measured by its ability to induce TNF-alpha secretion by J774A.1 mouse reticulum cell sarcoma macrophage cells.
  The ED50 for this effect is 1.5-7.5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 11.8 kDa
  SDS-PAGE: 11 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: Saa1 serum amyloid A 1 [ Mus musculus ]
• Official Symbol: Saa1
• Synonyms: SAA1; serum amyloid A 1; serum amyloid A-1 protein; serum amyloid A 2; Saa2; Saa-1
• Gene ID: 20208
• mRNA Refseq: NM_009117
• Protein Refseq: NP_033143
• UniProt ID: P05366

Reviews

06/05/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

09/21/2018

The product's manufacturer went above and beyond to ensure our satisfaction, strengthening our trust in the brand.

09/15/2018

The value derived from the product's usage made the investment worthwhile, considering the insights acquired.

Q&As

Are there specific stimuli or triggers that induce the expression and secretion of SAA1, influencing its role in the acute-phase response?

Specific stimuli or triggers, such as infection or tissue damage, induce the expression and secretion of SAA1, influencing its role in the acute-phase response.

What diseases or conditions are associated with dysregulation or abnormal expression of SAA1, especially in the context of inflammatory disorders?

Dysregulation or abnormal expression of SAA1 is associated with various inflammatory disorders, including rheumatoid arthritis and atherosclerosis.

In which cellular compartments is SAA1 predominantly synthesized and secreted?

SAA1 is predominantly synthesized in the liver and secreted into the bloodstream, serving as a major acute-phase protein.

What is the primary role of Serum Amyloid A1 (SAA1) in cellular processes and acute-phase responses?

Serum Amyloid A1 (SAA1) plays a crucial role in cellular processes, primarily contributing to acute-phase responses and inflammatory reactions.

How does SAA1 contribute to inflammation, particularly in the context of acute-phase reactions?

SAA1 contributes to inflammation by actively participating in acute-phase reactions, amplifying the immune response during various challenges.

Are there therapeutic implications or potential targets related to SAA1 in diseases involving acute-phase responses and chronic inflammation?

There are potential therapeutic implications related to SAA1 in diseases involving acute-phase responses and chronic inflammation, making it a target for drug development.

How does SAA1 participate in modulating immune responses and influencing the progression of inflammatory conditions?

SAA1 actively modulates immune responses, influencing the progression of inflammatory conditions and contributing to tissue repair.