Recombinant Human SAA1 293 Cell Lysate

Cat.No. : SAA1-2081HCL
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : Antigen standard for serum amyloid A1 (SAA1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SAA1 serum amyloid A1 [ Homo sapiens ]
Official Symbol : SAA1
Synonyms : SAA1; serum amyloid A1; SAA; serum amyloid A protein; PIG4; TP53I4; tumor protein p53 inducible protein 4; SAA2; MGC111216;
Gene ID : 6288
mRNA Refseq : NM_199161
Protein Refseq : NP_954630
MIM : 104750
UniProt ID : P02735
Chromosome Location : 11p15.1
Pathway : Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Amyloids, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Disease, organism-specific biosystem;
Function : G-protein coupled receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Reviews
  • Q&As

Customer Reviews (3)

Write a review
Reviews
06/05/2022

    Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

    09/21/2018

      The product's manufacturer went above and beyond to ensure our satisfaction, strengthening our trust in the brand.

      09/15/2018

        The value derived from the product's usage made the investment worthwhile, considering the insights acquired.

        Q&As (7)

        Ask a question
        Are there specific stimuli or triggers that induce the expression and secretion of SAA1, influencing its role in the acute-phase response? 11/24/2021

        Specific stimuli or triggers, such as infection or tissue damage, induce the expression and secretion of SAA1, influencing its role in the acute-phase response.

        What diseases or conditions are associated with dysregulation or abnormal expression of SAA1, especially in the context of inflammatory disorders? 12/15/2020

        Dysregulation or abnormal expression of SAA1 is associated with various inflammatory disorders, including rheumatoid arthritis and atherosclerosis.

        In which cellular compartments is SAA1 predominantly synthesized and secreted? 10/05/2020

        SAA1 is predominantly synthesized in the liver and secreted into the bloodstream, serving as a major acute-phase protein.

        What is the primary role of Serum Amyloid A1 (SAA1) in cellular processes and acute-phase responses? 09/10/2017

        Serum Amyloid A1 (SAA1) plays a crucial role in cellular processes, primarily contributing to acute-phase responses and inflammatory reactions.

        How does SAA1 contribute to inflammation, particularly in the context of acute-phase reactions? 02/26/2017

        SAA1 contributes to inflammation by actively participating in acute-phase reactions, amplifying the immune response during various challenges.

        Are there therapeutic implications or potential targets related to SAA1 in diseases involving acute-phase responses and chronic inflammation? 01/21/2017

        There are potential therapeutic implications related to SAA1 in diseases involving acute-phase responses and chronic inflammation, making it a target for drug development.

        How does SAA1 participate in modulating immune responses and influencing the progression of inflammatory conditions? 03/05/2016

        SAA1 actively modulates immune responses, influencing the progression of inflammatory conditions and contributing to tissue repair.

        Ask a Question for All SAA1 Products

        Required fields are marked with *

        My Review for All SAA1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends