Active Recombinant Human BMP2K Protein, N-His-tagged

• Cat.No.: BMP2K-28H
• Product Overview: N-terminal 6Histagged, recombinant fragment of human BIKe expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: This gene is the human homolog of mouse BMP-2-inducible kinase. Bone morphogenic proteins (BMPs) play a key role in skeletal development and patterning. Expression of the mouse gene is increased during BMP-2 induced differentiation and the gene product is a putative serine/threonine protein kinase containing a nuclear localization signal. Therefore, the protein encoded by this human homolog is thought to be a protein kinase with a putative regulatory role in attenuating the program of osteoblast differentiation. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Form: 2.41mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 20U/mg, where one unit of BIKe activity is defined as 1nmol phosphate incorporated into 250μM AP2tide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 44 kDa
• Purity: Purity 99% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BMP2K BMP2 inducible kinase [ Homo sapiens (human) ]
• Official Symbol: BMP2K
• Synonyms: BMP2K; BMP2 inducible kinase; BIKE; HRIHFB2017
• Gene ID: 55589
• mRNA Refseq: NM_017593.5
• Protein Refseq: NP_060063.2
• MIM: 617648
• UniProt ID: Q9NSY1