| Description : |
Fibroblast growth factor (FGF) superfamily consists of 23 members, all of which contain a conserved 120 amino acid (aa) core region that contains six identical, interspersed amino acids. Based on the pI factor, two main groups of FGF are named: acidic FGF (aFGF) and basic FGF (bFGF). The superfamily members act extracellularly through four tyrosine kinase FGF receptors (FGFR1 through FGFR4), with multiple specificities noted for almost all FGFs. Biological binding of aFGF to its receptors will result in phosphorylation of the receptor and non-receptor proteins and generate its function. FGFR4 (FGFR-4, Fibroblast growth factor receptor 4) is expressed mainly in lung, kidney, pancreas, spleen and developing muscle as well as overexpressed in some human malignancies where it has been implicated in their pathogenesis and progression. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
442-755 aa |
| Form : |
0.46mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1289U/mg, where one unit of FGFR4, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
36.4 kDa |
| AA Sequence : |
1 MHHHHHHEFG PALLAGLVSL DLPLDPLWEF PRDRLVLGKP LGEGCFGQVV RAEAFGMDPA 61 RPDQASTVAV KMLKDNASDK DLADLVSEME VMKLIGRHKN IINLLGVCTQ EGPLYVIVEC 121 AAKGNLREFL RARRPPGPDL SPDGPRSSEG PLSFPVLVSC AYQVARGMQY LESRKCIHRD 181 LAARNVLVTE DNVMKIADFG LARGVHHIDY YKKTSNGRLP VKWMAPEALF DRVYTHQSDV 241 WSFGILLWEI FTLGGSPYPG IPVEELFSLL REGHRMDRPP HCPPELYGLM RECWHAAPSQ 301 RPTFKQLVEA LDKVLLAVSE EYL |
| Purity : |
Purity 75% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
