"RAD51" Related Products

Expand

Recombinant Human RAD51, His-tagged

Cat.No.: RAD51-128H
Product Overview: The recombinant Human RAD51, His-tagged protein was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.
Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Source: E. coli
Species: Human
Tag: His
Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
Bio-activity: 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
Molecular Mass: 39.1 kDa.
AA Sequence: MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD
Purity: Greater than 90% as determined by SDS-PAGE.
Applications: RAD51 has been applied in DNA and protein-protein interactions assays.
Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: RAD51
Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog) , RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) , RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad51; HsT16930; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; RAD51 homolog (RecA homolog, E. coli); BRCA1/BRCA2-containing complex, subunit 5; RECA; MRMV2; HRAD51; RAD51A;
Gene ID: 5888
mRNA Refseq: NM_002875
Protein Refseq: NP_002866
MIM: 165160
UniProt ID: P05412
Chromosome Location: 1p32-p31
Pathway: ATF-2 transcription factor network, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem;
Function: DNA binding; R-SMAD binding; RNA polymerase II activating transcription factor binding; RNA polymerase II distal enhancer sequence-specific DNA binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; Rho GTPase activator activity; cAMP response element binding; double-stranded DNA binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription coactivator activity; transcription factor binding; transcription regulatory region DNA binding;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.