Recombinant Human F2

• Cat.No.: F2-170H
• Product Overview: Recombinant human F2 protein was expressed in E.coli.

Specification

• Description: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.
• Source: E.coli
• Species: Human
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol.
• Molecular Mass: 33.9kDa (296aa)
• AA Sequence: MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE
• Purity: > 80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: F2 coagulation factor II (thrombin) [ Homo sapiens (human) ]
• Official Symbol: F2
• Synonyms: F2; coagulationfactor II (thrombin); PT; prothrombin;serine protease; OTTHUMP00000197117; OTTHUMP00000233661; prothrombin B-chain
• Gene ID: 2147
• mRNA Refseq: NM_000506
• Protein Refseq: NP_000497
• MIM: 176930
• UniProt ID: P00734
• Chromosome Location: 11p11-q12
• Pathway: Angiopoietinreceptor Tie2-mediated signaling; BloodClotting Cascade; Cell surfaceinteractions at the vascular wall; CommonPathway; Diabetes pathways; G alpha (q) signalling events; GPCR downstream signaling
• Function: calciumion binding; growth factor activity; peptidase activity; protein binding;receptor binding; contributes_to receptor binding; serine-type endopeptidaseactivity; thrombospondin receptor activity