Active Recombinant Mouse Sema5a Protein, Fc Chimera

• Cat.No.: Sema5a-617M
• Product Overview: Recombinant Mouse Sema5a (Accession # NP_033180), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Glu23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.The ED50 for this effect is typically 0.9 - 3.6 µg/mL.
• Molecular Mass: Recombinant Mouse Sema5a, Fc Chimera has a calculated MW of 110.8 kDa (monomer). In SDS-PAGE migrates as 120-140 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Sema5a sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A [ Mus musculus ]
• Official Symbol: Sema5a
• Synonyms: SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; semaphorin-5A; sema F; M-Sema D; semaphorin F; semaphorin-F; semF; Semaf; AI464145; 5930434A13; 9130201M22Rik
• Gene ID: 20356
• mRNA Refseq: NM_009154
• Protein Refseq: NP_033180