||Highly pure (>98%) recombinant human sRANKL.
||This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
||Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
||Formulized from 5mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
||Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|Sequences of amino acids:
||hsRANK-L is a soluble 20.0 kDa protein, comprising the full-length of the TNF-like extracellular domain of RANKL:MEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSW YHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRH HETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGST KYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSL LDPDQDATYFGAFKVRDID
||Belongs to the tumor necrosis factor family.