Recombinant Human TNFSF11

• Cat.No.: TNFSF11-31288TH
• Product Overview: Highly pure (>98%) recombinant human sRANKL.

Specification

• Description: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
• Source: E. coli
• Tissue specificity: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
• Form: Lyophilised
• Storage buffer: Formulized from 5mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: hsRANK-L is a soluble 20.0 kDa protein, comprising the full-length of the TNF-like extracellular domain of RANKL:MEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSW YHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRH HETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGST KYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSL LDPDQDATYFGAFKVRDID
• Sequence Similarities: Belongs to the tumor necrosis factor family.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• Uniprot ID: O14788
• Chromosome Location: 13q14
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem; Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem
• Function: cytokine activity; cytokine activity; receptor activity; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding