Recombinant Human ALOX15 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALOX15-4696H
• Product Overview: ALOX15 MS Standard C13 and N15-labeled recombinant protein (NP_001131) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 74.6 kDa
• AA Sequence: MGLYRIRVSTGASLYAGSNNQVQLW LVGQHGEAALGKRLWPARGKETELK VEVPEYLGPLLFVKLRKRHLLKDDA WFCNWISVQGPGAGDEVRFPCYRWV EGNGVLSLPEGTGRTVGEDPQGLFQ KHREEELEERRKLYRWGNWKDGLIL NMAGAKLYDLPVDERFLEDKRVDFE VSLAKGLADLAIKDSLNVLTCWKDL DDFNRIFWCGQSKLAERVRDSWKED ALFGYQFLNGANPVVLRRSAHLPAR LVFPPGMEELQAQLEKELEGGTLFE ADFSLLDGIKANVILCSQQHLAAPL VMLKLQPDGKLLPMVIQLQLPRTGS PPPPLFLPTDPPMAWLLAKCWVRSS DFQLHELQSHLLRGHLMAEVIVVAT MRCLPSIHPIFKLIIPHLRYTLEIN VRARTGLVSDMGIFDQIMSTGGGGH VQLLKQAGAFLTYSSFCPPDDLADR GLLGVKSSFYPQDALRLWEIIYRYV EGIVSLHYKTDVAVKDDPELQTWCR EITEIGLQGAQDRGFPVSLQARDQV CHFVTMCIFTCTGQHASVHLGQLDW YSWVPNAPCTMRLPPPTTKDATLET VMATLPNFHQASLQMSITWQLGRRQ PVMVAVGQHEEEYFSGPEPKAVLKK FREELAALDKEIEIRNAKLDMPYEY LRPSVVENSVAITRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALOX15 arachidonate 15-lipoxygenase [ Homo sapiens (human) ]
• Official Symbol: ALOX15
• Synonyms: ALOX15; arachidonate 15-lipoxygenase; 15 LOX 1; 15-LOX; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; 15LOX-1; 15-LOX-1
• Gene ID: 246
• mRNA Refseq: NM_001140
• Protein Refseq: NP_001131
• MIM: 152392
• UniProt ID: P16050

Reviews

12/19/2022

Good fluorescence and absorption properties.

11/26/2021

ALOX15 has good stability in different environments and is not easily degraded.

08/26/2021

The effects of the recombinant proteins on the pathogenesis of related diseases were evaluated by animal models or in vitro experiments and found to be very good.

03/11/2021

The catalytic activity of ALOX15 protein is very excellent, and it can efficiently promote the progress of chemical reactions, showing excellent catalytic performance.

Q&As

In what way does ALOX15 regulate cell proliferation?

It can regulate cell proliferation by regulating various cell signaling pathways and influencing cell cycle.

How does oxidative stress initiated by ALOX15 participate in immune response?

The oxidative stress response initiated by ALOX15 can induce cells to secrete a variety of cytokines, thus participating in the immune response.

What is the effect of ALOX15 and GSTP1 gene variation on chemotherapy response of BLCA patients?

ALOX15 and GSTP1 gene variants may affect how well BLCA patients respond to chemotherapy.

What effect does the loss of ALOX15 have on the body?

ALOX15 deficiency may lead to a variety of effects such as alveolar hyperdilation, cardiomyopathy, and metabolic syndrome.

Which cell signaling pathways are involved in ALOX15?

ALOX15 is involved in many cell signaling pathways, such as PI3K/AKT pathway, MAPK pathway, NF-κB pathway, etc.

What inflammatory reactions does ALOX15 participate in?

ALOX15 is involved in a variety of inflammatory reactions, such as asthma, arthritis, and skin inflammation.

What is the role of ALOX15 in asthma?

ALOX15 is involved in the pathogenesis of asthma, mediating airway stenosis and inflammation through the synthesis of leukotrienes.

What is the role of ALOX15 in tumorigenesis and development?

The effects of ALOX15 vary in different tumor types, such as acting as an inhibitor in colorectal cancer and promoting tumor cell growth and invasion in prostate cancer.

How does ALOX15 regulate apoptosis?

ALOX15 can affect apoptosis by regulating ROS levels and influencing cell signaling pathways.

What diseases do mutations in ALOX15 cause?

Mutations in ALOX15 have been linked to diseases such as asthma and Behcet's disease.

What is the role of ALOX15 in neurodevelopment?

This protein is involved in the process of neural development, such as promoting the growth of nerve cells and affecting brain development.

How does the oxidative stress response initiated by ALOX15 affect cell function?

Oxidative stress response initiated by ALOX15 produces a large number of ROS, which affects various cell functions, such as apoptosis and cell proliferation.

In what diseases does ALOX15 play a role?

It is involved in the pathogenesis of many diseases, such as asthma, rheumatoid arthritis, and skin inflammation.

What role does ALOX15 play in the function of keratinocytes?

It is involved in various functions such as keratinocyte differentiation and cell cycle regulation.