| Description : |
Insulin-like growth factor (IGF) binding to the IGF receptors initiates an intracellular signaling cascade that leads to changes in gene expression and cell biology. Although multiple receptors for the IGFs have been identified, the IGFs primarily exert their biologic effects through ligation of the IGF1R (type I IGF receptor tyrosine kinase, JTK13). IGF1R exerts mitogenic, cell survival, and insulin-like activities, is involved in postnatal growth physiology, and is connected to proliferative disorders such as breast cancer. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
959-end |
| Form : |
1.984mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution. |
| Bio-activity : |
3661U/mg, where one unit of IGF-1R activity is defined as 1nmol phosphate incorporated into 500μM IGFtide (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
48 kDa |
| AA Sequence : |
1 MAHHHHHHEN LYFQGHRKRN NSRLGNGVLY ASVNPEYFSA ADVYVPDEWE VAREKITMSR 61 ELGQGSFGMV YEGVAKGVVK DEPETRVAIK TVNEAASMRE RIEFLNEASV MKEFNCHHVV 121 RLLGVVSQGQ PTLVIMELMT RGDLKSYLRS LRPEMENNPV LAPPSLSKMI QMAGEIADGM 181 AYLNANKFVH RDLAARNCMV AEDFTVKIGD FGMTRDIYET DYYRKGGKGL LPVRWMSPES 241 LKDGVFTTYS DVWSFGVVLW EIATLAEQPY QGLSNEQVLR FVMEGGLLDK PDNCPDMLFE 301 LMRMCWQYNP KMRPSFLEII SSIKEEMEPG FREVSFYYSE ENKLPEPEEL DLEPENMESV 361 PLDPSASSSS LPLPDRHSGH KAENGPGPGV LVLRASFDER QPYAHMNGGR KNERALPLPQ 421 SSTC |
| Purity : |
Purity 88.3% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
