Active Recombinant Mouse IGF1R Protein, His-tagged

• Cat.No.: IGF1R-22M
• Product Overview: Recombinant Mouse IGF1R Protein (Met1-His936) was expressed in CHO cells with C-terminal His tag. This protein is covalently-linked heterotetramers (2 alpha and 2 beta subunits) & covalently-linked single chain homodimers.

Specification

• Source: CHO
• Species: Mouse
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA. When rmIGF-IR is present at 0.5 μg/mL, the concentration of rmIGF-I that produces 50% of the optimal binding response is found to be approximately 25-100 ng/mL.
• Molecular Mass: 104.5 kDa (single chain), 80.7 kDa ( alpha subunit) & 23.2 kDa ( beta subunit)
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Storage at -20 to -70 centigrade.
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Igf1r insulin-like growth factor I receptor [ Mus musculus ]
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor I receptor; insulin-like growth factor 1 receptor; line 186; IGF-I receptor; hyft; CD221; IGF-1R; D930020L01; A330103N21Rik
• Gene ID: 16001
• mRNA Refseq: NM_010513
• Protein Refseq: NP_034643

Reviews

07/08/2022

The product's exceptional purity facilitated precise quantification, ensuring data consistency.

06/14/2019

The product consistently delivered robust and dependable results, affirming its efficacy in our experiments.

04/29/2018

The product's consistent performance bolstered the dependability of our research conclusions.

Q&As

In which cellular compartments is IGF1R predominantly localized?

IGF1R is predominantly localized on the cell membrane, where it acts as a receptor for insulin-like growth factors (IGFs) and insulin.

Are there specific downstream signaling pathways activated by IGF1R, and how do they influence cell proliferation and survival?

Downstream signaling pathways activated by IGF1R include the Ras/MAPK pathway and PI3K/Akt pathway, contributing to cell growth and survival.

How does IGF1R interact with insulin-like growth factors (IGFs) and insulin, and what is its role in growth regulation?

IGF1R interacts with IGFs and insulin, triggering downstream signaling pathways such as the PI3K/Akt pathway, influencing cell proliferation and survival.

Are there therapeutic implications or potential targets related to IGF1R in diseases involving growth signaling?

There are potential therapeutic implications related to IGF1R in diseases involving growth signaling, making it a target for drug development.

What diseases or conditions are associated with dysregulation or abnormal expression of IGF1R?

Dysregulation or abnormal expression of IGF1R is associated with conditions such as cancer and developmental disorders.

What is the primary function of Insulin-like growth factor 1 receptor (IGF1R) in cellular processes and growth signaling?

Insulin-like growth factor 1 receptor (IGF1R) functions in cellular processes, primarily playing a role in growth signaling.

How does IGF1R contribute to the regulation of tissue development, particularly in the context of growth and differentiation?

IGF1R contributes to the regulation of tissue development by influencing cell growth, differentiation, and survival.