Active Recombinant Mouse Sema3c protein, His-tagged

• Cat.No.: Sema3c-902M
• Product Overview: Recombinant Mouse Sema3c protein (Gln24-Ser741), fused to His-tag at N-terminus, was expressed in NS0 cells.

Specification

• Description: This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein.
• Source: Insect cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid, NaCl and Tween 20.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.The ED50 for this effect is 0.12-1.2 μg/mL.
• Molecular Mass: 108.9 kDa
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Sema3c
• Official Symbol: Sema3c
• Synonyms: SemE; Semae; 1110036B02Rik
• Gene ID: 20348
• mRNA Refseq: NM_013657.5
• Protein Refseq: NP_038685.3
• UniProt ID: Q62181