Recombinant Human SEMA3C Protein, hIgG-tagged

• Cat.No.: SEMA3C-05H
• Product Overview: Recombinant human Semaphorin 3C (21-738aa), fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease.
• Source: HEK293
• Species: Human
• Tag: hIgG
• Form: Liquid
• Molecular Mass: 107.2 kDa (951aa)
• Protein length: 21-738aa
• AA Sequence: GSSQPQARVYLTFDELRETKTSEYF SLSHHPLDYRILLMDEDQDRIYVGS KDHILSLNINNISQEALSVFWPAST IKVEECKMAGKDPTHGCGNFVRVIQ TFNRTHLYVCGSGAFSPVCTYLNRG RRSEDQVFMIDSKCESGKGRCSFNP NVNTVSVMINEELFSGMYIDFMGTD AAIFRSLTKRNAVRTDQHNSKWLSE PMFVDAHVIPDGTDPNDAKVYFFFK EKLTDNNRSTKQIHSMIARICPNDT GGLRSLVNKWTTFLKARLVCSVTDE DGPETHFDELEDVFLLETDNPRTTL VYGIFTTSSSVFKGSAVCVYHLSDI QTVFNGPFAHKEGPNHQLISYQGRI PYPRPGTCPGGAFTPNMRTTKEFPD DVVTFIRNHPLMYNSIYPIHKRPLI VRIGTDYKYTKIAVDRVNAADGRYH VLFLGTDRGTVQKVVVLPTNNSVSG ELILEELEVFKNHAPITTMKISSKK QQLYVSSNEGVSQVSLHRCHIYGTA CADCCLARDPYCAWDGHSCSRFYPT GKRRSAAQDVRHGNPLTQCRGFNLK AYRNAAEIVQYGVKNNTTFLECAPK SPQASIKWLLQKDKDAAKEVKLNER IIATSQGLLIRSVQGSDQGLYHCIA TENSFKQTIAKINFKVLDSEMVAVV TDKWSPWTWASSVRALPFHPKDIMG AFSHSEMQMINQYCKDTRQQHQQGD ESQKMRGDYGKLKALINS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: SEMA3C semaphorin 3C [ Homo sapiens (human) ]
• Official Symbol: SEMA3C
• Synonyms: SEMA3C; semaphorin 3C; SemE; SEMAE; semaphorin-3C; sema E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C; semaphorin E
• Gene ID: 10512
• mRNA Refseq: NM_006379
• Protein Refseq: NP_006370.1
• MIM: 602645
• UniProt ID: Q99985