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Principle & Protocol

Principle and Protocol of Purification of GST Fusion Protein

Principle and Protocol of Purification of GST Fusion Protein

The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of glutathione S-transferase (GST) fusion protein, as well as the main operation steps and precautions.

Principle and Protocol of Prokaryotic Expression and Detection

Principle and Protocol of Prokaryotic Expression and Detection

The purpose of this experimental protocol is to help researchers mastering the basic principles and main steps of prokaryotic expression of the target protein, and master the basic steps and precautions of Coomassie brilliant blue staining and immunoblotting experiment.

Principle and Protocol of Silver Stain

Principle and Protocol of Silver Stain

Silver staining is an important dyeing method, which is widely used because of its low cost, safe reagents, fast and simple, and high sensitivity. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Silver Staining.

Principle and Protocol of Coomassie Brilliant Blue

Principle and Protocol of Coomassie Brilliant Blue

Coomassie brilliant blue staining method can reach the detection level of microgram level, and the linear dynamic correlation range between staining degree and protein content is wide, which is suitable for quantitative analysis. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Coomassie brilliant blue dyeing.

Principle and Protocol of Isoelectric Focusing Electrophoresis

Principle and Protocol of Isoelectric Focusing Electrophoresis

Isoelectric focusing electrophoresis usually adopts horizontal plate electrophoresis or tube electrophoresis. It has high resolution and sensitivity, and is particularly suitable for studying the microscopic heterogeneity of proteins. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of Isoelectric Focusing Electrophoresis.

Principle and Protocol of SDS-PAGE

Principle and Protocol of SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is the most commonly used electrophoretic technique for protein separation. SDS polyacrylamide gel electrophoresis is to add SDS. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of SDS-PAGE.

Principle and Protocol of Native-PAGE

Principle and Protocol of Native-PAGE

Polyacrylamide gel is mainly used for protein electrophoresis. Polyacrylamide gel electrophoresis is a process in which polyacrylamide gel is used as a support medium to separate the action of charged particles in an electric field. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of native-PAGE.

Principle and Protocol of Kjeldahl Method

Principle and Protocol of Kjeldahl Method

The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Kjeldahl method for determining protein concentration. Kjeldahl determination of nitrogen is a commonly used method for analyzing nitrogen content of organic compounds at present. It is one of the most accurate and simple methods for determining total organic nitrogen in samples, and has been taken as the standard inspection method determined internationally and domestically.

Principle and Protocol of BCA Method

Principle and Protocol of BCA Method

BCA method is one of the most commonly used protein concentration detection methods in the world at present. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of BCA method for determining protein concentration, and understand the test method and application of BCA method.

Principle and Protocol of Lowry Method

Principle and Protocol of Lowry Method

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Lowry method for determining protein concentration, and understand the test method and application of Lowry method.

Principle and Protocol of Biuret Method

Principle and Protocol of Biuret Method

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Biuret method for determining protein concentration, and understand the test method and application of Biuret method.

Principle and Protocol of Bradford Method

Principle and Protocol of Bradford Method

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Bradford method for determining protein concentration, and understand the test method and application of Bradford method.

Principle and Protocol of Protein Concentration Measurement by UV

Principle and Protocol of Protein Concentration Measurement by UV

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of UV method for determining protein concentration, and understand the test method and application of UV method.

Protocol of Preparation of Ubiquitinated Protein Samples

Protocol of Preparation of Ubiquitinated Protein Samples

Purpose of this handbook is help researchers to understand the ubiquitinated protein and their roles, and to master the basic strategies and main methods for enrichment of ubiquitinated protein.

Protocol of Preparation of Glycosylated Protein Samples

Protocol of Preparation of Glycosylated Protein Samples

Purpose of this handbook is help researchers to understand the glycosylated protein modifications and their roles, and to master the basic strategies and main methods for enrichment of glycosylated protein.

Protocol of Preparation of Phosphorylated Protein Samples

Protocol of Preparation of Phosphorylated Protein Samples

Phosphorylation of proteins is one of the most important covalent modifications in organisms. Purpose of this handbook is help researchers to understand the phosphorylated Protein and their roles, and to master the basic strategies and main methods for enrichment of phosphorylated proteins.

Protocol of Preparation of the Subcellular Organelle Protein Samples

Protocol of Preparation of the Subcellular Organelle Protein Samples

The purpose of this handbook is to help researchers to master the main methods and corresponding precautions for the separation of animal and plant organelles, master the preparation methods of subcellular protein samples and understand the importance of subcellular proteomics.

Protocol of Preparation of the Total Protein Samples

Protocol of Preparation of the Total Protein Samples

Proteins exist in complex mixtures in tissues or cells. The purpose of this handbook is to help researchers to master the general principle of protein sample preparation, and design the experimental process of total protein sample preparation in combination with the experimental purpose."

X-ray Crystallography

X-ray Crystallography

X-ray crystallography is a technique used for determining the atomic and molecular structure of a crystal.

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