Active Recombinant Full Length Human PRKCI Protein, N-His-tagged

• Cat.No.: PRKCI-151HFL
• Product Overview: N-terminal 6Histagged, recombinant full-length human PKC iota expressed in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Protein kinase C, iota (PKCiota, PRKCI) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.453mg/ml of enzyme in 20mM HEPES/NaOH pH7.4, 250mM NaCl, 50% glycerol, 5mM DTT, 2mM EDTA, 2mM EGTA, 0.05% Triton X-100. Frozen solution.
• Bio-activity: 828U/mg, where one unit of PKC iota, active activity is defined as 1nmol phosphate incorporated into 50μM PKCtide (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 68.6 kDa
• AA Sequence: 1 MHHHHHHEFM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF 61 DNEQLFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE 121 DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK 181 KCHKLVTIEC GRHSLPQEPV MPMDQSSMHS DHAQTVIPYN PSSHESLDQV GEEKEAMNTR 241 ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ 301 TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA 361 EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI 421 APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR 481 IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK 541 PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV
• Purity: Purity 98.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCI protein kinase C iota [ Homo sapiens (human) ]
• Official Symbol: PRKCI
• Synonyms: PRKCI; protein kinase C iota; PKCI; DXS1179E; nPKC-iota
• Gene ID: 5584
• mRNA Refseq: NM_002740.6
• Protein Refseq: NP_002731.4
• MIM: 600539
• UniProt ID: P41743