Active Recombinant Human PTPN22(1-312) Protein, N-GST-tagged

• Cat.No.: PTPN22-209H
• Product Overview: N-terminal GSTtagged, recombinant, human PTPN22, amino acids 1–312, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: PTPN22 (Protein Tyrosine Phosphatase Non-Receptor Type 22) is a member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. This protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 1-312 aa
• Form: 0.43mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 12806U/mg, where one unit of PTPN22 activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 63.3 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM DQREILQKFL 241 DEAQSKKITK EEFANEFLKL KRQSTKYKAD KTYPTTVAEK PKNIKKNRYK DILPYDYSRV 301 ELSLITSDED SSYINANFIK GVYGPKAYIA TQGPLSTTLL DFWRMIWEYS VLIIVMACME 361 YEMGKKKCER YWAEPGEMQL EFGPFSVSCE AEKRKSDYII RTLKVKFNSE TRTIYQFHYK 421 NWPDHDVPSS IDPILELIWD VRCYQEDDSV PICIHCSAGC GRTGVICAID YTWMLLKDGI 481 IPENFSVFSL IREMRTQRPS LVQTQEQYEL VYNAVLELFK RQMDVIRDKH SGTESQAKHC 541 I
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTPN22 protein tyrosine phosphatase non-receptor type 22 [ Homo sapiens (human) ]
• Official Symbol: PTPN22
• Synonyms: PTPN22; protein tyrosine phosphatase non-receptor type 22; LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
• Gene ID: 26191
• mRNA Refseq: NM_012411.6
• Protein Refseq: NP_036543.5
• MIM: 600716
• UniProt ID: Q9Y2R2