Active Recombinant Human PTPN4(423-end) Protein, N-GST-tagged

• Cat.No.: PTPN4-207H
• Product Overview: N-terminal GSTtagged, recombinant, human PTP MEG1, amino acids 423–end, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: PTPMEG-1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in Signaling downstream of the glutamate receptors through tyrosine dephosphorylation.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 423-end
• Form: 1.09mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 239U/mg, where one unit of PTP MEG1 activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 83.1 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMVHT SPSEVFVNQR 241 SPSSTQANSI VLESSPSQET PGDGKPPALP PKQSKKNSWN QIHYSHSQQD LESHINETFD 301 IPSSPEKPTP NGGIPHDNLV LIRMKPDENG RFGFNVKGGY DQKMPVIVSR VAPGTPADLC 361 VPRLNEGDQV VLINGRDIAE HTHDQVVLFI KASCERHSGE LMLLVRPNAV YDVVEEKLEN 421 EPDFQYIPEK APLDSVHQDD HSLRESMIQL AEGLITGTVL TQFDQLYRKK PGMTMSCAKL 481 PQNISKNRYR DISPYDATRV ILKGNEDYIN ANYINMEIPS SSIINQYIAC QGPLPHTCTD 541 FWQMTWEQGS SMVVMLTTQV ERGRVKCHQY WPEPTGSSSY GCYQVTCHSE EGNTAYIFRK 601 MTLFNQEKNE SRPLTQIQYI AWPDHGVPDD SSDFLDFVCH VRNKRAGKEE PVVVHCSAGI 661 GRTGVLITME TAMCLIECNQ PVYPLDIVRT MRDQRAMMIQ TPSQYRFVCE AILKVYEEGF 721 VKPLTTSTNK
• Purity: Purity 56% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTPN4 protein tyrosine phosphatase non-receptor type 4 [ Homo sapiens (human) ]
• Official Symbol: PTPN4
• Synonyms: PTPN4; protein tyrosine phosphatase non-receptor type 4; MEG; PTPMEG; PTPMEG1
• Gene ID: 5775
• mRNA Refseq: NM_002830.4
• Protein Refseq: NP_002821.1
• MIM: 176878
• UniProt ID: P29074