Recombinant Human PTPN4, GST-tagged

• Cat.No.: PTPN4-603H
• Product Overview: Recombinant human catalytic domain (amino acids 637-926) of MEG-1 with N-terminal GST tag was expressed in an E. coli expression system. MW = 60 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 637-926 a.a.
• Description: Tyrosine-protein phosphatase non-receptor type 4 is an enzyme that in humans is encoded by the PTPN4 gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
• Specific Activity: 15,000 U/mg.1 unit of MEG-1 activity is equal to 1 nmol phosphate hydrolyzed from p-nitrophenyl phosphate substrate per minute at pH 7.4 and 30 ºC.
• Assay Condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP substrate, and 5 µg/ml recombinant MEG-1.
• Purity: > 95% by SDS-PAGE.
• Formulation: Recombinant protein is in storage buffer (25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 2 mM EDTA, 1 mM DTT, and 50% glycerol).
• Application: MEG-1 is useful for the study of enzyme kinetics, regulation, target substrate dephosphorylation, and inhibitor screening.
• Storage And Stability: Store product frozen at or below -70 ºC. Enzyme will be stable for at least 6 months at -70 ºC as undiluted stock. Freeze in working aliquots to avoid repeated thawing and freezing.

Gene Information

• Gene Name: PTPN4 protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte) [ Homo sapiens ]
• Synonyms: protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte); PTPMEG; PTPMEG1; PTPN4; tyrosine-protein phosphatase non-receptor type 4; MEG; PTPase-MEG1; megakaryocyte phosphatase; protein tyrosine phosphatase MEG1; protein-tyrosine phosphatase MEG1; megakaryocyte protein-tyrosine phosphatase; EC 3.1.3.48
• Gene ID: 5775
• mRNA Refseq: NM_002830
• Protein Refseq: NP_002821
• MIM: 176878
• UniProt ID: P29074
• Chromosome Location: 2q14.2
• Function: non-membrane spanning protein tyrosine phosphatase activity; cytoskeletal protein binding; hydrolase activity; protein binding