Active Recombinant Human ITGA5, Strep II-His-tagged

• Cat.No.: ITGA5-5282H
• Product Overview: Recombinant human Integrin alpha 5 beta 1 heterodimer protein (Human ITGA5 & ITGB1) Phe 42 - Tyr 995 (Integrin alpha 5, NP_002196) and Gln 21 - Asp 728 (Integrin beta 1, NP_002202) was co-expressed in human 293 cells (HEK293).

Specification

• Description: Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions. Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions.
• Source: HEK293 Cells
• Species: Human
• Tag: Strep II-His
• Predicted N Terminal: Phe42 (Integrin alpha 5) and Gln21 (Integrin beta 1)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Recombinant Human alpha 5 beta 1 binds rhFibronectin coated at 2 μg/ml with an apparent KD< 0.4 nm in the presence of 1 mm>
• Molecular Mass: 105.4 kDa ( alpha 5 subunit), 79.2 kDa ( beta 1 subunit). DTT-reduced Protein migrates as 105-150 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Human ITGA5 & ITGB1 by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

Gene Information

• Gene Name: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) [ Homo sapiens ]
• Official Symbol: ITGA5
• Synonyms: ITGA5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); FNRA; integrin alpha-5; CD49e; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit; VLA5A
• Gene ID: 3678
• mRNA Refseq: NM_002205
• Protein Refseq: NP_002196
• MIM: 135620
• UniProt ID: P08648
• Chromosome Location: 12q11-q13
• Pathway: Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Axon guidance, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem
• Function: integrin binding; platelet-derived growth factor receptor binding; protein binding; receptor activity; vascular endothelial growth factor receptor 2 binding