Active Recombinant Human CDC42BPG(1-473) Protein, N-Flag,N-His-tagged

• Cat.No.: CDC42BPG-159H
• Product Overview: N-terminal FLAGtagged and N-terminal 6His-tagged, recombinant, human MRCKγ amino acids 1-473 expressed by baculovirus in Sf21 insect cells. Purified using immunoaffinity chromatography.

Specification

• Description: MRCKg enables ATP binding activity; magnesium ion binding activity; and protein serine/threonine kinase activity. MRCKg is also involved in protein phosphorylation. Located in cell leading edge; centriolar satellite; and cytosol.
• Source: Sf21
• Species: Human
• Tag: Flag,His
• Protein length: 1-473 aa
• Form: 0.58mg/ml of enzyme in 43mM Tris/HCl pH7.5, 129mM NaCl, 270mM sucrose, 129μg/ml FLAG peptide, 0.9mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 193U/mg, where one unit of MRCKγ activity is defined as 1nmol phosphate incorporated into 50μM KKRNRTLTV per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 56 kDa
• AA Sequence: 1 MGGDYKDDDD KDPHHHHHHM ERRLRALEQL ARGEAGGCPG LDGLLDLLLA LHHELSSGPL 61 RRERSVAQFL SWASPFVSKV KELRLQRDDF EILKVIGRGA FGEVTVVRQR DTGQIFAMKM 121 LHKWEMLKRA ETACFREERD VLVKGDSRWV TTLHYAFQDE EYLYLVMDYY AGGDLLTLLS 181 RFEDRLPPEL AQFYLAEMVL AIHSLHQLGY VHRDVKPDNV LLDVNGHIRL ADFGSCLRLN 241 TNGMVDSSVA VGTPDYISPE ILQAMEEGKG HYGPQCDWWS LGVCAYELLF GETPFYAESL 301 VETYGKIMNH EDHLQFPPDV PDVPASAQDL IRQLLCRQEE RLGRGGLDDF RNHPFFEGVD 361 WERLASSTAP YIPELRGPMD TSNFDVDDDT LNHPGTLPPP SHGAFSGHHL PFVGFTYTSG 421 SHSPESSSEA WAALERKLQC LEQEKVELSR KHQEALHAPT DHRELEQLRK EVQTLRDRLP 481 EMLRDKASLS QT
• Purity: Purity 99% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDC42BPG CDC42 binding protein kinase gamma [ Homo sapiens (human) ]
• Official Symbol: CDC42BPG
• Synonyms: CDC42BPG; CDC42 binding protein kinase gamma; DMPK2; MRCKG; HSMDPKIN; KAPPA-200; MRCKgamma
• Gene ID: 55561
• mRNA Refseq: NM_017525.3
• Protein Refseq: NP_059995.2
• MIM: 613991
• UniProt ID: Q6DT37