Active Recombinant Human DUSP10(320-end) Protein, N-His-tagged

• Cat.No.: DUSP10-150H
• Product Overview: N-terminal 6Histagged, recombinant, human MKP5, amino acids 320–end, expressed in E. coli cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MKP5, also known as DUSP10 (Dual Specificity Phosphatase 10), is a dual specificity protein phosphatase that inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotryosine residues. Dual specificity protein phosphatases negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: 320-end
• Form: 2.16mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1511U/mg, where one unit of MKP5, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8- difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 20.6 kDa
• AA Sequence: 1 MHHHHHHENL YFQGAELTPI LPFLFLGNEQ DAQDLDTMQR LNIGYVINVT THLPLYHYEK 61 GLFNYKRLPA TDSNKQNLRQ YFEEAFEFIE EAHQCGKGLL IHCQAGVSRS ATIVIAYLMK 121 HTRMTMTDAY KFVKGKRPII SPNLNFMGQL LEFEEDLNNG VTPRILTPKL MGVETVV
• Purity: Purity 94% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DUSP10 dual specificity phosphatase 10 [ Homo sapiens (human) ]
• Official Symbol: DUSP10
• Synonyms: DUSP10; dual specificity phosphatase 10; MKP5; MKP-5
• Gene ID: 11221
• mRNA Refseq: NM_007207.6
• Protein Refseq: NP_009138.1
• MIM: 608867
• UniProt ID: Q9Y6W6