Active Recombinant Human FES(2-end) Protein, N-His-tagged

Cat.No. : FES-80H
Product Overview : N-terminal 6Histagged recombinant human Fes, residues 2–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.
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Description : The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S transition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
Source : Sf21
Species : Human
Tag : His
Protein length : 2-end
Form : 1.18mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Bio-activity : 83U/mg, where one unit of Fes, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
Molecular Mass : 98 kDa
AA Sequence : 1 MSYYHHHHHH DYDIPTTENL YFQGAMGIRN SGFSSELCSP QGHGVLQQMQ EAELRLLEGM 61 RKWMAQRVKS DREYAGLLHH MSLQDSGGQS RAISPDSPIS QSWAEITSQT EGLSRLLRQH 121 AEDLNSGPLS KLSLLIRERQ QLRKTYSEQW QQLQQELTKT HSQDIEKLKS QYRALARDSA 181 QAKRKYQEAS KDKDRDKAKD KYVRSLWKLF AHHNRYVLGV RAAQLHHQHH HQLLLPGLLR 241 SLQDLHEEMA CILKEILQEY LEISSLVQDE VVAIHREMAA AAARIQPEAE YQGFLRQYGS 301 APDVPPCVTF DESLLEEGEP LEPGELQLNE LTVESVQHTL TSVTDELAVA TEMVFRRQEM 361 VTQLQQELRN EEENTHPRER VQLLGKRQVL QEALQGLQVA LCSQAKLQAQ QELLQTKLEH 421 LGPGEPPPVL LLQDDRHSTS SSEQEREGGR TPTLEILKSH ISGIFRPKFS LPPPLQLIPE 481 VQKPLHEQLW YHGAIPRAEV AELLVHSGDF LVRESQGKQE YVLSVLWDGL PRHFIIQSLD 541 NLYRLEGEGF PSIPLLIDHL LSTQQPLTKK SGVVLHRAVP KDKWVLNHED LVLGEQIGRG 601 NFGEVFSGRL RADNTLVAVK SCRETLPPDL KAKFLQEARI LKQYSHPNIV RLIGVCTQKQ 661 PIYIVMELVQ GGDFLTFLRT EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL 721 VTEKNVLKIS DFGMSREEAD GVYAASGGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL 781 WETFSLGASP YPNLSNQQTR EFVEKGGRLP CPELCPDAVF RLMEQCWAYE PGQRPSFSTI 841 YQELQSIRKR HRKLVEKY
Purity : Purity 83% by SDS-PAGE and Coomassie blue staining.
Applications : Drug Discovery & Development
Storage : 1 year at -70 centigrade.
Handling Advice : Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
Use/Stability : On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
FACS: 2.3–29.5ng of this lot of enzyme phosphorylated 0.1mg/ml poly(Glu, Tyr) (4:1) in the assay.
Gene Name : FES FES proto-oncogene, tyrosine kinase [ Homo sapiens (human) ]
Official Symbol : FES
Synonyms : FES; FES proto-oncogene, tyrosine kinase; FPS
Gene ID : 2242
mRNA Refseq : NM_002005.4
Protein Refseq : NP_001996.1
MIM : 190030
UniProt ID : P07332

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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