Active Recombinant Human PIK3C2G(468-1203) Protein, N-GST-tagged

• Cat.No.: PIK3C2G-192H
• Product Overview: N-terminal GSTtagged recombinant human PI3K-C2γ amino acids 468–1203, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: PI3K-C2γ protein belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The biological function of this gene has not yet been determined.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 468-1203 aa
• Form: 0.406mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 953U/mg, where one unit of PI3K-C2γ, active activity is defined as 1nmol phosphatidylinositol 3-phosphate (PI(3)P) formed per minute at room temperature with a final ATP concentration of 200μM.
• Molecular Mass: 112 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRHQSSE 241 TSAKGLIEKV TTELSTSIYQ LINVYCNSFY ADFQPVNVPR CTSYLNPGLP SHLSFTVYAA 301 HNIPETWVHR INFPLEIKSL PRESMLTVKL FGIACATNNA NLLAWTCLPL FPKEKSILGS 361 MLFSMTLQSE PPVEMITPGV WDVSQPSPVT LQIDFPATGW EYMKPDSEEN RSNLEEPLKE 421 CIKHIARLSQ KQTPLLLSEE KKRYLWFYRF YCNNENCSLP LVLGSAPGWD ERTVSEMHTI 481 LRRWTFSQPL EALGLLTSSF PDQEIRKVAV QQLDNLLNDE LLEYLPQLVQ AVKFEWNLES 541 PLVQLLLHRS LQSIQVAHRL YWLLKNAENE AYFKSWYQKL LAALQFCAGK ALNDEFSKEQ 601 KLIKILGDIG ERVKSASDHQ RQEVLKKEIG RLEEFFQDVN TCHLPLNPAL CIKGIDHDAC 661 SYFTSNALPL KITFINANLM GKNISIIFKA GDDLRQDMLV LQLIQVMDNI WLQEGLDMQM 721 IIYRCLSTGK DQGLVQMVPD AVTLAKIHRH SGLIGPLKEN TIKKWFSQHN HLKADYEKAL 781 RNFFYSCAGW CVVTFILGVC DRHNDNIMLT KSGHMFHIDF GKFLGHAQTF GGIKRDRAPF 841 IFTSEMEYFI TEGGKNPQHF QDFVELCCRA YNIIRKHSQL LLNLLEMMLY AGLPELSGIQ 901 DLKYVYNNLR PQDTDLEATS HFTKKIKESL ECFPVKLNNL IHTLAQMSAI SPAKSTSQTF 961 PQESCLLSTT RS
• Purity: Purity 70.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIK3C2G phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma [ Homo sapiens (human) ]
• Official Symbol: PIK3C2G
• Synonyms: PIK3C2G; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma; PI3K-C2GAMMA; PI3K-C2-gamma
• Gene ID: 5288
• mRNA Refseq: NM_004570.6
• Protein Refseq: NP_004561.3
• MIM: 609001
• UniProt ID: O75747